1 La mayora de los organismos vivos no pueden ser teidos debido a que los colorantes utilizados pueden daar su estructura celular hasta el punto de su muerte. CONCLUSIONS: These results suggest that NCI-N87 cell line may be useful in H. Microscopio de contraste de fases El microscopio de contraste de fases permite observar clulas sin colorear y resulta especialmente til para clulas vivas. las tcnicas de Microscopa ptica de luz reflejada (MOLR), Microscopa electrnica de. In NCI-N87 cell lines, IL-8 level was significantly increased after 24 hour compared to control, and was prominent in the lower well. Microscopa ptica (campo claro, contraste de fases, campo. TER was measured as 400-500 (omega x cm2) at two or three weeks after cell culture. O microscpio de contraste de fase segue a mesma estrutura bsica do microscpio. A utilizao do contraste de fase se d por no necessitar corar as clulas, mas mesmo assim proporcionar a visualizao detalhada da amostra. RESULTS: A monolayer phenotype was observed in NCI-N87 cell lines by using confocal microscope. Na microscopia de campo claro comum, analisar clulas vivas pode ser mais complicado pela falta de contraste. IL-8 level was compared in both upper and lower well with the control. The IL-8 level was determined by ELISA at 24 hours after the administration of HP60190 and IL-1alpha on NCI-N87 cells. Transepithelial resistance (TER) was measured on NCI-N87 cells seeded on Transwell(R) to identify monolayer polarity two or three times a week after confluency. METHODS: For the identification of epithelial phenotypes at low magnification, cells were observed on a phase-contrast microscope and confocal microscope. The present investigation aimed at assessing the usefulness of NCI-N87 cell line as an adequate cellular model to study the pathophysiology of human H. BACKGROUND/ AIMS: The unavailability of human gastric cell lines representative of the normal gastric epithelial function such as polarized monolayer restricts the application of cell culture system in approaching the field of Helicobacter pylori (H. Dessa forma pretendemos apresentar fotografias feitas no microscpio, em cmeras, em lupas, tcnicas de colorao, fixao, corte e preparo de lminas para a.
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